An FBI analyst has testified that DNA sampled from a rifle, as well as multiple other items, found near where President Donald Trump was playing golf in South Florida last year matches that of a man accused of trying to assassinate Trump that day

This courtroom sketch shows Secret Service agent Robert Fercano identifying the defendant in the trial of Ryan Routh, who is charged with attempting to assassinate Donald Trump last year at a golf course in South Florida, Thursday, Sept. 11, 2025, in Fort Pierce, Fla. (Lothar Speer via AP)

DNA sampled from a rifle, as well as multiple other items, found near where President Donald Trump was playing golf in South Florida last year matches that of a man accused of trying to assassinate Trump that day, an FBI analyst testified Tuesday.

Tuesday was the fourth day of testimony in the trial of Ryan Routh, who prosecutors said spent weeks plotting to kill Trump before aiming a rifle through the shrubbery as Trump played golf on Sept. 15, 2024, at his West Palm Beach country club.

Routh has pleaded not guilty to charges of attempting to assassinate a major presidential candidate, assaulting a federal officer and several firearm violations.

U.S. District Judge Aileen Cannon had initially blocked off more than three weeks for trial at the Fort Pierce federal courthouse. But prosecutors have said they should be able to rest their case by Thursday, and Routh’s witnesses have been subpoenaed to appear by Friday.

Prosecutors continued to call expert witnesses on Tuesday, according to local news outlets. A ballistics expert testified about two metal plates found mounted to the golf course fence, which would have been nearly impossible for handgun rounds to penetrate. Investigators believe Routh had planned to use the plates for cover.

One analyst testified that Routh was a potential DNA contributor to more than two dozen items collected from the crime scene, including an SKS rifle. Another expert described how Routh’s Google and Facebook accounts were logged in to several phones recovered from his SUV and contained location data that tracked his movements over the weeks leading up to the attempted attack.

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This courtroom sketch shows Secret Service agent Robert Fercano holding up the weapon found in the bushes during opening statements in the trial of Ryan Routh, who is charged with attempting to assassinate Donald Trump last year at a golf course in South Florida, Thursday, Sept. 11, 2025, in Fort Pierce, Fla. (Lothar Speer via AP)

Routh has indicated that he plans to call a firearms expert, as well as several character witnesses. He hasn’t said whether he plans to testify himself.

Recounting the incident at the Trump International Golf Club, a Secret Service agent testified last week that he spotted Routh before Trump came into view. Routh aimed his rifle at the agent, who opened fire, causing Routh to drop his weapon and flee without firing a shot.

Law enforcement obtained help from a witness who testified that he saw a person fleeing the area after hearing gunshots. The witness was then flown in a police helicopter to a nearby interstate where Routh was arrested, and the witnesses said he confirmed it was the person he had seen.

Just nine weeks earlier, Trump had survived an attempt on his life while campaigning in Pennsylvania. That gunman had fired eight shots, with one bullet grazing Trump’s ear, before being fatally shot by a Secret Service counter sniper.

This courtroom sketch shows lead government prosecutor John Shipley speaking during the opening statements of the during the trial of Ryan Routh, who is charged with attempting to assassinate Donald Trump last year at a golf course in South Florida, Thursday, Sept. 11, 2025, in Fort Pierce, Fla. (Lothar Speer via AP)

A pickup truck decorated in support of President Donald Trump sits parked outside the Alto Lee Adams Sr. U.S. Courthouse, after the start of jury selection in the trial of Ryan Routh, charged with trying to assassinate Trump while he played golf last year in South Florida, Monday, Sept. 8, 2025, in Fort Pierce, Fla. (AP Photo/Rebecca Blackwell)

A law enforcement officer from the Department of Homeland Security stands watch at the Alto Lee Adams Sr. U.S. Courthouse as jury selection began in the trial of Ryan Routh, charged with trying to assassinate Donald Trump while he played golf last year in South Florida, Monday, Sept. 8, 2025, in Fort Pierce, Fla. (AP Photo/Rebecca Blackwell)